Guideline Regarding Peds/Winship Flow Cytometry Core Operations during Phase-2 Research Ramp-up effective from 8/13/2020 (Stay tuned for updates)
The Pediatric/Winship Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning. The Flow Cytometry Core offers access to several analytical flow cytometers as well as high-speed cell sorting. Training and technical expertise is available to enable our users to improve the quality and scope of their research. Contact Aaron Rae for any questions. The Flow Cytometry Core also offers immunological-based assay services, please contact core director David Archer for questions. Flow Core resource page for grants, downloadable document.
The core is located in E-362 in the Heath Science Research Building and is accessible to researchers 24/7 by keycard, and instruments can be booked via the PPMS website. There are no food or drinks permitted in the Flow Core.
Our facility is also located in Winship Building C, Room C-5027 and is accessible to researchers from 7am-7pm Mon to Fri by keycard. Instruments can be booked via the PPMS link above.
To request access to Building C please submit the following form: Winship Access Request
The Flow Cytometry Core offers access to several analytical flow cytometers and Luminex as well as high-speed cell sorting. We also offer training as well as expert help to enable our users to improve the quality and scope of their research.
The facility provides the following list of services:
The core supports a wide range of applications, please contact a member of staff for further information.
To Register for an account in the Pediatrics/Winship Flow Cytometry Core please click here.
Bookings can be made online for the analyzers, and the Luminex through PPMS. Once users have been trained they will be given access to book the equipment online. The calendars are located at: https://ppms.us/emory/?ECFCC
PLEASE NOTE: If you move labs or leave Emory please let the Flow Core know as soon as possible so we can update your record.
If you have mulitple Projects or SPEEDTYPES that you wish to bill your flow charges to you can request that separate projects be created. This allows for organization of hours booked per project for billing purposes. Please visit: PPMS Project Request
Pricing (Janurary 2020)
- Cell Sorting, ARIA II - $130/hr
- Cell Sorting, SONY - $79/hr (unassisted)
- Imagestream - $37/hr
- Analyzers, LSR II, Auroras, Symphony & Cytoflex - $37/hr
- Sample Processing/Data Analysis/Service Consultation - $51/hr
- Offline Analysis Workstations - $0/hr
Pediatric researchers are eligible for subsidized rates on selected services. Winship members doing cancer projects are eligible for subsidized rates. Please contact David Archer to determine eligibility. An additional fee will be charged for service consultation, please contact David Archer for details.
In order to keep the Pediatrics/Winship Flow Cytometry Core instruments in good working order for all users, detailed use instructions and cleaning procedures are included in the instrument standard operating procedures (SOP). When a user makes deviations from the SOP that result in instrument downtime or inhibits the next user from typical use, the user at fault may be charged to facilitate necessary repairs. This may include, but is not limited to, part replacement and technician repair/troubleshoot time. If you have questions about how to properly use or maintain the instrument to keep it in proper working order, please do not hesitate to ask Aaron Rae or Lisa Bixby. If you find an instrument to be malfunctioning or not working at its best, please immediately report the issue, this will prevent further damage and properly identify the problem.
Any equipment cancellations made 24 hours or more in advance to the booking will be honored and no charges will be assessed to the user. Any equipment cancellations made LESS THAN 24 hours in advance to the booking will be charged in full to the investigator’s discretionary (i.e. non-sponsored) account. For additional details on the cancellation policy, please see the Flow Cytometry Core Billing Practices Subsidies and Cancelation Policies (PDF).
Generous subsidies are offered for those investigators who are actively engaging in child health-related or for Winship Cancer Institute members doing cancer-related projects research. Subsidies will only be applied to the amount of time actually used on the machine, rather than the time booked. The investigator will be responsible for paying the full rate for any time that was booked and not used. For additional details on the subsidy policy, please see the Flow Cytometry Core Billing Practices Subsidies and Cancelation Policies (PDF).
Flow Cytometry Core Training Policy
Complementary training for independent equipment use is provided for all users who are active users of the Flow Cytometry Core. Allocations are as follows:
Three 1.5 hour training sessions for analyzer use (including LSR, Canto and Cytoflex)
Three 1.5 hour training sessions for Sony Sorter use
Only personal assistance time is complimentary; equipment usage during training is billed at the full rate.
Additional training time exceeding these allocations will be charged at the assisted rate.
If trained users do not make use of the equipment they were trained upon within 3 months of their last training date, they will be retroactively charged for all hours used.
Flow Cytometry Core Assisted Sorting Fee Policy
Use of the Sony Sorter is considered an unassisted service. If users request equipment set up or other assistance, they will be charged accordingly for Flow Core personnel time.
Flow Cytometry Core Assisted Sorting Policy
Users are expected to turn up at their scheduled time, if you are running late please inform the member of staff you have booked with. Failure to inform a member of staff no later than 30 minutes after the scheduled booking time will result in an automatic cancellation of the booking and the user being charged the full amount*.
*exteunating circumstances permitted at the discretion of the Technical Director.
BSL2 safety practices should be employed in the facility. Primary human cells or cells exposed to recombinant virus should be fixed before analysis on the instruments.
All users are required to fill our the Flow Cytometry Facility Cell Sorting Form prior to their sort being performed.
As of the May 1, 2012 all users that are requesting cell sorting are required to download the Cell Sorting Guidelines from the EHSO. A Risk Assesment must be provided to the Flow Cytometry Facility before any sorting can be performed, a template is provided within the EHSO guidelines.
The Flow Cytometry Core is generously supported by Children's Healthcare of Atlanta, Winship Cancer Institute, and Emory University. When presenting or publishing work completed using the core, please include "Pediatric/Winship Flow Cytometry Core" in the acknowledgments.
To determine if you should use "Children's Healthcare of Atlanta" in your author affiliations, please see guidelines here.
Reporting Instrument Issues
The Emory + Pediatrics/Winship flow cytometry core is pleased to announce that we can offer remote support on some of our instruments.
If a staff member is available they will provide you with a zoom link for you to click on, this will open up a Zoom Meeting and allow two way communication via camera/microphone as well as screen sharing and control.
To request support:
Submit a Low priority Incident Report as usual via PPMS.
IF a staff member is available they will email you a link to click on within 5 minutes. (If you do not receive a link within this timeframe then staff are not available).
A remote session will open, you may be requested to give permission for a member of staff to share and control the screen, they will walk you through the steps.
We hope that this will provide a quicker way to assist users to resolve most issues without an in person visit.
Support is available on the following instruments:
FACSymphony A5 – Orac (https://ppms.us/emory/creatinc/?item=178)
FACSymphony A3 – Liberator (https://ppms.us/emory/creatinc/?item=190)
Cytek Aurora – Zen (https://ppms.us/emory/creatinc/?item=179)
Cytek Aurora – Avon (https://ppms.us/emory/creatinc/?item=180)
BC Cytoflex - The Hooded Claw
Imagestream (ISX) – The Buzzwagon
FACS Aria II – The Mean Machine (for live viewing of your sort)