Giglio et al. article in ACS: Infectious Diseases

"Transcriptomic Profiling Suggests That Promysalin Alters the Metabolic Flux, Motility, and IronRegulation in Pseudomonas putida KT2440." Giglio KM, Keohane CE, Stodghill PV, Steele AD, Fetzer C, Sieber SA, Filiatrault MJ, Wuest WM. ACS Infect Dis. 2018 Jun 5.

Full article


Promysalin, a secondary metabolite produced by P. putida RW10S1, is a narrow-spectrum antibiotic that targets P. aeruginosa over other Pseudomonas spp. P. putida KT2440, a nonproducing strain, displays increased swarming motility and decreased pyoverdine production in the presence of exogenous promysalin. Herein, proteomic and transcriptomic experiments were used to provide insight about how promysalinelicits responses in PPKT2440 and rationalize its species selectivity. RNA-sequencing results suggest that promysalin affects PPKT2440 by (1) increasing swarming in a flagella-independent manner; (2) causing cells to behave as if they were experiencing an iron-deficient environment, and (3) shifting metabolism away from glucose conversion to pyruvate via the Entner-Doudoroff pathway. These findings highlight nature's ability to develop small molecules with specific targets, resulting in exquisite selectivity.