The Pediatric/Winship Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning. The Flow Cytometry Core offers access to several analytical flow cytometers (9), an imaging cytometer as well as high-speed cell sorters (2). Training and technical expertise is available to enable our users to improve the quality and scope of their research. Contact Aaron Rae for any questions. The Flow Cytometry Core also offers immunological-based assay services, please contact core director David Archer for questions. Our welcome document provides all of the information you need to access and use the core.
What is Flow Cytometry?
How does a Flow Cytometer Work?
The core is located in E-362 in the Heath Science Research Building and is accessible to researchers 24/7 by keycard, and instruments can be booked via the PPMS website. There are no food or drinks permitted in the Flow Core.
Our facility is also located in Winship Building C, Room C-5027, Winship Building B, Room B3202 and is accessible to researchers from 7am-7pm Mon to Fri by keycard. Instruments can be booked via the PPMS link above.
To request access to Building B&C please submit the following form: Winship Access Request
To Register for an account in the Pediatrics/Winship Flow Cytometry Core please click here.
Bookings can be made online for the analyzers through PPMS. Once users have been trained they will be given access to book the equipment online. The calendars are located at: https://ppms.us/emory/?ECFCC
PLEASE NOTE: If you move labs or leave Emory please let the Flow Core know as soon as possible so we can update your record.
Projects
If you have mulitple Projects or SPEEDTYPES that you wish to bill your flow charges to you can request that separate projects be created. This allows for organization of hours booked per project for billing purposes. Please visit: PPMS Project Request
BSL2 safety practices should be employed in the facility. Primary human cells or cells exposed to recombinant virus should be fixed before analysis on the instruments.
All users are required to fill out the Flow Cytometry Facility Cell Sorting Form prior to their sort being performed.
As of the May 1, 2012 all users that are requesting cell sorting are required to download the Cell Sorting Guidelines from the EHSO. A Risk Assessment must be provided to the Flow Cytometry Facility before any sorting can be performed, a template is provided within the EHSO guidelines.
Core Usage Statistics - Updated 6th October 2025
* YTD
** Hours Booked
*** Number of Training Sessions
Instrument**/Service | 2020 | 2021 | 2022 | 2023 | 2024 | 2025 |
---|---|---|---|---|---|---|
Instrument Training*** | 166 | 198 | 167 | 219 | 226 | 148 |
Foundations Training*** (Jan 2025) | 0 | 0 | 0 | 0 | 0 | 130 |
BD FACSymphony A5 Orac | 1956 | 1812 | 1548 | 1460 | 1895 | 1048 |
BD FACSymphony A3 Liberator | 962 | 1659 | 1681 | 1268 | 1209 | 948 |
BD FACSymphony A1 Villa (Oct 2022) | 0 | 0 | 81 | 487 | 757 | 677 |
Cytek Aurora Zen | 1890 | 2175 | 1158 | 803 | 1599 | 1160 |
Cytek Aurora Avon | 1198 | 1556 | 967 | 1389 | 1750 | 1552 |
Cytek Aurora Blake (July 2021) | 0 | 353 | 1031 | 1469 | 2310 | 1600 |
Cytek Aurora Soolin (Jan 2022) | 0 | 0 | 596 | 1238 | 1408 | 1048 |
FACS Aria II (Ret Sep 2024) | 534 | 703 | 700 | 664 | 368 | 0 |
Aurora CS (Online May 2023) | 0 | 0 | 0 | 161 | 433 | 328 |
BD FACSDiscover S8 (Nov 2024) | 0 | 0 | 0 | 0 | 12 | 151 |
Cytoflex The Hooded Claw | 1097 | 1395 | 777 | 618 | 351 | 231 |
Imagestream MKII Buzzwagon | 47 | 83 | 35 | 44 | 233 | 96 |
Total | 7684 | 9736 | 8574 | 9601 | 12325 | 8839 |
The FACSDiscover S8 and Aurora CS (previously ARIA II) are regularly tested (every three months) for Aerosol containment and Mycoplasma. The results from these tests will be posted here shortly after. If one of these tests fails, corrective action will be taken.
- Results May 26th, 2021
- Results August 24th 2021
- Results September 1st 2021 (Repeat)
- Results September 3rd 2021 (Repeat)
- Results October 27th 2021
- Results April 10th 2023
- Results August 10th 2023
- Results November 15th 2023
- Results February 22nd 2024
- Results May 14th 2024
- Results August 16th 2024
- Results November 14th 2024