Flow Cytometry Core

Pediatric/Winship Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning.

PPMS Bookings and Online Registration

The Pediatric/Winship Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning. The Flow Cytometry Core offers access to several analytical flow cytometers (9), an imaging cytometer as well as high-speed cell sorters (2). Training and technical expertise is available to enable our users to improve the quality and scope of their research. Contact Aaron Rae for any questions. The Flow Cytometry Core also offers immunological-based assay services, please contact core director David Archer for questions. Our welcome document provides all of the information you need to access and use the core.

What is Flow Cytometry?
How does a Flow Cytometer Work?

The core is located in E-362 in the Heath Science Research Building and is accessible to researchers 24/7 by keycard, and instruments can be booked via the PPMS website. There are no food or drinks permitted in the Flow Core.

Our facility is also located in Winship Building C, Room C-5027, Winship Building B, Room B3202 and is accessible to researchers from 7am-7pm Mon to Fri by keycard. Instruments can be booked via the PPMS link above.

To request access to Building B&C please submit the following form: Winship Access Request

Registration

To Register for an account in the Pediatrics/Winship Flow Cytometry Core please click here.

Bookings can be made online for the analyzers through PPMS. Once users have been trained they will be given access to book the equipment online. The calendars are located at: https://ppms.us/emory/?ECFCC

PLEASE NOTE: If you move labs or leave Emory please let the Flow Core know as soon as possible so we can update your record.

Projects

If you have mulitple Projects or SPEEDTYPES that you wish to bill your flow charges to you can request that separate projects be created. This allows for organization of hours booked per project for billing purposes. Please visit: PPMS Project Request

Biosafety

BSL2 safety practices should be employed in the facility. Primary human cells or cells exposed to recombinant virus should be fixed before analysis on the instruments.

All users are required to fill out the Flow Cytometry Facility Cell Sorting Form prior to their sort being performed.

As of the May 1, 2012 all users that are requesting cell sorting are required to download the Cell Sorting Guidelines from the EHSO. A Risk Assessment must be provided to the Flow Cytometry Facility before any sorting can be performed, a template is provided within the EHSO guidelines.

Core Statistics

Core Usage Statistics - Updated 6th October 2025

* YTD
** Hours Booked
*** Number of Training Sessions

Instrument**/Service	2020	2021	2022	2023	2024	2025
Instrument Training***	166	198	167	219	226	148
Foundations Training*** (Jan 2025)	0	0	0	0	0	130
BD FACSymphony A5 Orac	1956	1812	1548	1460	1895	1048
BD FACSymphony A3 Liberator	962	1659	1681	1268	1209	948
BD FACSymphony A1 Villa (Oct 2022)	0	0	81	487	757	677
Cytek Aurora Zen	1890	2175	1158	803	1599	1160
Cytek Aurora Avon	1198	1556	967	1389	1750	1552
Cytek Aurora Blake (July 2021)	0	353	1031	1469	2310	1600
Cytek Aurora Soolin (Jan 2022)	0	0	596	1238	1408	1048
FACS Aria II (Ret Sep 2024)	534	703	700	664	368	0
Aurora CS (Online May 2023)	0	0	0	161	433	328
BD FACSDiscover S8 (Nov 2024)	0	0	0	0	12	151
Cytoflex The Hooded Claw	1097	1395	777	618	351	231
Imagestream MKII Buzzwagon	47	83	35	44	233	96
Total	7684	9736	8574	9601	12325	8839

Testing Results

The FACSDiscover S8 and Aurora CS (previously ARIA II) are regularly tested (every three months) for Aerosol containment and Mycoplasma. The results from these tests will be posted here shortly after. If one of these tests fails, corrective action will be taken.

Meet the Team

Upcoming Events

Tue Oct 2111:00AM-12:00PM