Joshua D. Chandler, PhD
Assistant Professor of Pediatrics
Pulmonology, Allergy/Immunology, CF, & Sleep
My lab focuses on cystic fibrosis (CF) and the roles of myeloperoxidase, an enzyme that produces an array of oxidants and free radicals, in the disease. In addition, we study the diverging roles of oxidants generated during inflammation (by enzymes like myeloperoxidase) and how an increase of one at the expense of another may cause changes in biological and health outcomes (a phenomenon I call “oxidant switching”). Major goals include identifying mechanisms of myeloperoxidase release by neutrophils in CF airways; characterizing myeloperoxidase activity in the CF airway environment; profiling the impact of myeloperoxidase on patient health; and identifying ways oxidant switching and other interventions may protect lung tissue from irreversible damage. My lab uses high-resolution, accurate mass metabolomics of basic and clinical samples to detect metabolites related to inflammatory processes. We also emphasize state-of-the-art biochemical analysis of proteins such as myeloperoxidase, and use rigorous approaches to analyzing redox state in basic and clinical samples. Additional areas of study include metabolomics of asthma and leukemia.
BA, Biology and Chemistry, Drury University, 2009
PhD, Molecular Toxicology, University of Colorado, 2014; PI: Brian J. Day, PhD
Postdoctoral Fellowship, Emory University, 2014-2017; PIs: Dean P. Jones, PhD and Young-Mi Go, PhD