The Pediatric Flow Cytometry Core provides cytometry services for the analysis and sorting of cells as well as expert consultation for experimental design and planning. The Flow Cytometry Core offers access to several state of the art analytical flow cytometers as well as high-speed cell sorting. Training and technical expertise is available to enable our users to improve the quality and scope of their research. Contact Aaron Rae or Bridget Neary for questions. The Flow Cytometry Core also offers immunological-based assay services, please contact core director David Archer for questions.
The core is located in E-362 in the Heath Science Research Building and is accessible to researchers 24/7 by keycard.
The Pediatric Flow Cytometry Core offers access to several state of the art analytical flow cytometers and Luminex as well as high-speed cell sorting. We also offer training as well as expert help to enable our users to improve the quality and scope of their research.
The facility provides flow cytometric analyzers and Luminex for the following applications:
- Cell Cycle
- Mitochondrial Potential
- Cell Proliferation
- Oxidative Burst
- Cytokine leveins in serum and plasma
- Gene and protein expression in cells and body fluids
To Register for an account in the Pediatric Flow Cytometry Core please click here.
Bookings can be made online for the analyzers, Luminex and the autoMACS Pro through PPMS. Once users have been trained they will be given access to book the equipment online. The calendars are located at: https://ppms.info/pedsflowcore/
The following links provide excellent web based training to aid you in getting the most out of your flow cytometry experiments:
To request training please login to PPMS, choose the Request Tab and select the appropriate choice for training.
Pricing (1st April 2016)
- Cell Sorting, ARIA II - $154/hr
- Cell Sorting, SONY - $107/hr (unassisted)
- Imagestream - $98/hr
- Analyzers, LSR - $30/hr
- Luminex - $72 to run a whole plate, $36 for a half plate
- Offline Analysis Workstation - $0/hr
Pediatric researchers are eligible for subsidized rates. Please contact Dr. David Archer to determine eligibility. An additional fee will be charged for service consultation, please contact Dr. David Archer for details.
In order to keep the Pediatric Flow Cytometry Core instruments in good working order for all users, detailed use instructions and cleaning procedures are included in the instrument standard operating procedures (SOP). When a user makes deviations from the SOP that result in instrument downtime or inhibits the next user from typical use, the user at fault may be charged to facilitate necessary repairs. This may include, but is not limited to, part replacement and technician repair/troubleshoot time. If you have questions about how to properly use or maintain the instrument to keep it in proper working order, please do not hesitate to ask Aaron Rae or Bridget Neary. If you find an instrument to be malfunctioning or not working at its best, please immediately report the issue, this will prevent further damage and properly identify the problem.
Any equipment cancellations made 24 hours or more in advance to the booking will be honored and no charges will be assessed to the user. Any equipment cancellations made LESS THAN 24 hours in advance to the booking will be charged in full to the investigator’s discretionary (i.e. non-sponsored) account. For additional details on the cancellation policy, please see the Billing Policy PDF.
Generous subsidies are offered for those investigators who are actively engaging in Pediatric research. Subsidies, however, will only be applied to the amount of time actually used on the machine, rather than the time booked. The investigator will be responsible for paying the full rate for any time that was booked and not used. For additional details on the subsidy policy, please see the pdf above.
Pediatric Flow Cytometry Core Training Policy
Complementary training for independent equipment use is provided for all users who are active users of the Pediatric Flow Cytometry Core. Allocations are as follows:
Three 1.5 hour training sessions for analyzer use (including LSR, Canto and Cytoflex)
Three 1 hour training sessions for Sony Sorter use
Only personal assistance time is complimentary; equipment usage during training is billed at the full rate.
Additional training time exceeding these allocations will be charged at the assisted rate.
If trained users do not make use of the equipment they were trained upon within 3 months of their last training date, they will be retroactively charged for all hours used.
Pediatric Flow Cytometry Core Assisted Sorting Fee Policy
Use of the Sony Sorter is considered an unassisted service. If users request equipment set up or other assistance, they will be charged accordingly for Pediatric Flow Core personnel time.
Access to instrumentation will be granted after training or an induction has been completed.
Flow Cytometry Core Equipment
- BD FACS Canto II Flow Cytometer - Lab E-362, HSRB
- BD LSRII Flow Cytometer - Lab E-362, HSRB
- BD LSRII Flow Cytometer - Lab E-362, HSRB
- BD FACS Aria II Cell Sorter - Lab E-362A, HSRB
- Imagestream X Mark II - Lab E-362, HSRB
- Miltenyi AutoMACS Pro - Whitehead, RM 655
- Luminex 100 Analyzer - Lab E-362, HSRB
- CTL-ImmunoSpot-S6 Micro Analyzer (ELISPOT Reader) - E-480, HSRB
Instrument manuals can be viewed here.
Instrument Standard Filter Set*
* Not all filters are suitable for all fluorochromes, please speak to a member of the facility if you require different or more optimal filters.
BSL2 safety practices should be employed in the facility. Primary human cells or cells exposed to recombinant virus should be fixed before analysis on the instruments.
All users are required to download, fill out and bring the Pediatric Research Alliance Flow Cytometry Facility Biosafety Form to the Cell Sorting Facility prior to their sort being performed.
As of the May 1, 2012 all users that are requesting cell sorting are required to download the Cell Sorting Guidelines from the EHSO. A Risk Assesment must be provided to the Flow Cytometry Facility before any sorting can be performed, a template is provided within the EHSO guidelines.
The Flow Cytometry Core is generously supported by Children's Healthcare of Atlanta and Emory University. When presenting or publishing work completed using the core, please include "Children's Healthcare of Atlanta and Emory University Pediatric Flow Cytometry Core" in the acknowledgments.
To determine if you should use "Children's Healthcare of Atlanta" in your author affiliations, please see guidelines here.