Overview

The Biomarkers Core provides state of the art equipment and up-to-date technology to provide high quality analysis of biological samples in the fastest turnaround time possible to support pediatric research. This core provides equipment and technical expertise to assay samples using methods that combine the features of gas-liquid chromatography and mass spectrometry.

The Biomarkers Core is located in the Emory-Children's Center in Room 310. When you have identified your project and needs, please contact Dr. Lou Ann Brown directly to discuss sample collection, preservation and analysis.

Core description for use in NIH Facilities & Resources page:

The Pediatric Biomarkers Core facility provides the equipment and technical expertise to assay samples using methods that combine the features of gas-liquid chromatography and mass spectrometry. These core services are applicable to a wide variety of sample types and will allow small-molecule metabolite profile identification. The Core has an Agilent gas chromatography/mass spectrometer and a Waters High Performance Liquid Chromatography with fluorescence detector. The Biomarkers Core currently performs analysis of biomarkers of oxidative stress including reduced and oxidized glutathione, cysteine, cystine, isoprostanes, hydroxynonenals, and malonyldialdehydes. This core also analyzes Fatty Acid Ethyl Esters (FAEE) from biological samples such as meconium, hair, placenta, blood, plasma as markers of alcohol use and exposure. The core is located in the Emory Children’s Center Research building.

The Biomarkers Core currently performs analysis of biomarkers of oxidative stress including reduced and oxidized glutathione, cysteine, cystine, isoprostanes, hydroxynonenals, and malonyldialdehydes. This core also analyzes Fatty Acid Ethyl Esters (FAEE) from biological samples such as meconium, hair, placenta, blood, and plasma as markers of alcohol use and exposure. The core will work with investigators to develop protocols to suit their needs.

Current analyses performed by the Biomarkers Core:

  • Biomarkers of oxidative stress including reduced and oxidized glutathione, cysteine, cystine, isoprostanes, hydroxynonenal, and malonyldialdehyde
  • Fatty Acid Ethyl Esters (FAEE) from biological samples such as meconium, hair, placenta, blood, and plasma as markers of alcohol use and exposure
  • Intracellular levels of ribonucleoside triphosphates and deoxynucleoside triphosphates, useful in the study of cellular metabolism and nucleic acid synthesis
  • Creatine levels in cells and urine for the study of creatine disorders

Pricing is per sample and does not include protocol development. An intake meeting will outline all fees and necessary protocol development.

  • HPLC processing is $53 per sample.
  • GC/MS and LC/MS/MS processing is $147 per sample.
  • Other services to analyze biomarkers may be available. Contact Dr. Lou Ann Brown to discuss feasibility.

Pediatric researchers may be eligible for subsidized rates.  Please contact Dr. Lou Ann Brown to determine eligibility.

The Biomarkers Core includes equipment to perform High Performance Liquid Chromatography (HPLC), High Performance Liquid Chromatography/Mass Spectroscopy (HPLC/MS) and Gas Chromatography/Mass Spectroscopy (GC/MS). Specific equipment includes:

  • Waters High Performance Liquid Chromatography with Fluorescence Detector
  • HP Agilent Gas Chromatograph/Mass Spectrometer
  • Thermo Finnigan TSQ Quantum Triple Quad LC/MS/MS
  • Other analyte analysis available, including ELISA, colorimetric, or fluorometric assays, contact core director to inquire

The Biomarkers Core is generously supported by Children's Healthcare of Atlanta and Emory University. When presenting or publishing work completed using the core, please include "Children's Healthcare of Atlanta and Emory University Pediatric Biomarkers Core" in the acknowledgments.

To determine if you should use "Children's Healthcare of Atlanta" in your author affiliations, please see guidelines here.