Next Generation Normalization for Western Blots
Location: HSRB E-360 Seminar HSRB E-363 Demo
Advancements in Western Blotting Technology: Bio-Rad's Western Workflow delivers more sensitive results, faster at a lower cost.
Current Challenges of Western Blotting techniques:
• Achieve quantitative interpretation of western blot data in terms of fold changes in protein expression
• No built in check points, researchers can waste time and money repeating experiments
• No quick and reliable method to determine loading controls and housekeeping protein assessment
• Most loading control proteins are highly abundant and not in the same range as our protein of interest
Bio-Rad’s V3 system enables you to address these issues:
• Visualize and quantify proteins in gels and blots without any staining/de-staining protocols
• Includes built in QC checks to measure protein separation, quality and efficiency of transfer, and validation
• Faster, more reliable loading control/normalization as compared to housekeeping protein method
• Significantly reduce time and cost while accuracy of western data is improved!